GenomeRNAi - a database for RNAi phenotypes and reagents
TitleScreen TitleAssayBiomodelSpecies
High-content siRNA screening of the kinome identifies kinases involved in Alzheimer's disease-related tau hyperphosphorylation. Azorsa et al. (2010) tau phosphorylation Total tau and 12E8 tau protein expressionH4 overexpressing 4RON tauH. sapiens


Neurofibrillary tangles (NFT), a cardinal neuropathological feature of Alzheimer''s disease (AD) that is highly correlated with synaptic loss and dementia severity, appear to be partly attributable to increased phosphorylation of the microtubule stabilizing protein tau at certain AD-related residues. Identifying the kinases involved in the pathologic phosphorylation of tau may provide targets at which to aim new AD-modifying treatments.

Screen details

Stable Id: GR00143-A
Screen title: tau phosphorylation
Assay: Total tau and 12E8 tau protein expression
Method: Fluorescence
Scope: Kinases
Screen type: Cell-based
Species: Homo sapiens
Biosource: Cell line
Biomodel: H4 overexpressing 4RON tau
Library: Qiagen, Validated human kinase siRNA Set 2.0
Reagent type: siRNA
Score type: p-value
Cutoff: Complex criteria