GenomeRNAi - a database for RNAi phenotypes and reagents
TitleScreen TitleAssayBiomodelSpecies
Whole genome functional analysis identifies novel components required for mitotic spindle integrity in human cells. Rines et al. (2008) Mitotic spindle integrity Histone H3 phosphorylation, alpha-tubulin protein expression and DNA contentHeLaH. sapiens

Abstract

The mitotic spindle is a complex mechanical apparatus required for accurate segregation of sister chromosomes during mitosis. We designed a genetic screen using automated microscopy to discover factors essential for mitotic progression. Using a RNA interference library of 49,164 double-stranded RNAs targeting 23,835 human genes, we performed a loss of function screen to look for small interfering RNAs that arrest cells in metaphase.

Screen details


Stable Id: GR00099-A
Screen title: Mitotic spindle integrity
Assay: Histone H3 phosphorylation, alpha-tubulin protein expression and DNA content
Method: Fluorescence
Scope: Genome-wide
Screen type: Cell-based
Species: Homo sapiens
Biosource: Cell line
Biomodel: HeLa
Library: Qiagen, np
Reagent type: siRNA
Score type: Mitotic index
Cutoff: >= 3 standard deviations above mean
Notes: Ontology-based pattern identification (OPI) algorithm used.