GenomeRNAi - a database for RNAi phenotypes and reagents
TitleScreen TitleAssayBiomodelSpecies
Identification of host proteins required for HIV infection through a functional genomic screen. Brass et al. (2008) HIV-1 infection (2) Tat-dependent β-Gal reporterTZM-blH. sapiens

Abstract

HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependency factors (HDFs). These proteins participate in a broad array of cellular functions and implicate new pathways in the viral life cycle. Further analysis revealed previously unknown roles for retrograde Golgi transport proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediator complex (Med28) in viral transcription. Transcriptional analysis revealed that HDF genes were enriched for high expression in immune cells, suggesting that viruses evolve in host cells that optimally perform the functions required for their life cycle. This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy.

Screen details


Stable Id: GR00163-A-2
Screen title: HIV-1 infection (2)
Assay: Tat-dependent β-Gal reporter
Method: Luminescence
Scope: Genome-wide
Screen type: Cell-based
Species: Homo sapiens
Biosource: Cell line
Biomodel: TZM-bl
Library: Dharmacon, siARRAY siRNA Library
Reagent type: siRNA
Score type: Relative light units
Cutoff: >= 2 standard deviations below plate mean
Notes: