GenomeRNAi - a database for RNAi phenotypes and reagents
TitleScreen TitleAssayBiomodelSpecies
Identification of host factors involved in borna disease virus cell entry through a small interfering RNA functional genetic screen. Clemente et al. (2010) Borna disease virus infection ViabilityOlH. sapiens

Abstract

Borna disease virus (BDV), the prototypic member of the Bornaviridae family, within the order Mononegavirales, is highly neurotropic and constitutes an important model system for the study of viral persistence in the central nervous system (CNS) and associated disorders. The virus surface glycoprotein (G) has been shown to direct BDV cell entry via receptor-mediated endocytosis, but the mechanisms governing cell tropism and propagation of BDV within the CNS are unknown. We developed a small interfering RNA (siRNA)-based screening to identify cellular genes and pathways that specifically contribute to BDV G-mediated cell entry. Our screen relied on silencing-mediated increased survival of cells infected with rVSVDeltaG*/BDVG, a cytolytic recombinant vesicular stomatitis virus expressing BDV G that mimics the cell tropism and entry pathway of bona fide BDV. We identified 24 cellular genes involved in BDV G-mediated cell entry. Identified genes are known to participate in a broad range of distinct cellular functions, revealing a complex process associated with BDV cell entry. The siRNA-based screening strategy we have developed should be applicable to identify cellular genes contributing to cell entry mediated by surface G proteins of other viruses.

Screen details


Stable Id: GR00192-A
Screen title: Borna disease virus infection
Assay: Viability
Method: Luminescence
Scope: Druggable genes
Screen type: Cell-based
Species: Homo sapiens
Biosource: Cell line
Biomodel: Ol
Library: Ambion, Silencer Human Druggable Genome siRNA Library V2
Reagent type: siRNA
Score type: Fold change
Cutoff: >= 2 for at least 2 siRNAs
Notes: Additional information about secondary screens (rVSVĪ”G*/VSVG infection, etc.)